Who changed the anneal step?
Benchmark records who changed a protocol, when, and why.
PCR, 30 cycles
Protocols, molecular
- v1
- v2
- v3
- v4
- v5
| Step | Temperature | Time |
|---|---|---|
| 1Initial denaturation | 95 °C | 3 min |
| 2Denature | 95 °C | 30 s |
| 3Anneal | 30 s | |
| 4Extend | 72 °C | 1 min |
| 5Final extension | 72 °C | 5 min |
- Who
- Scientist
- When
- 2026-10-09 09:41:07
- Version
- v4 to v5
- Why
- “Primer dimers at 58 °C.”
100 V
90V
Every value keeps its reason
- Where
- Western blot transfer, step 2
- Who
- Lab manager
- When
- 2026-10-08 16:05:12
- Why
- “Bands smeared at 100 V.”
What did it say before?
Every version is kept. Read any two side by side.
| Change | Step | v4 2 Oct, Scientist | v5 9 Oct, Scientist |
|---|---|---|---|
| 1Initial denaturation | 95 °C, 3 min | 95 °C, 3 min | |
| 2Denature | 95 °C, 30 s | 95 °C, 30 s | |
| ± | 3Anneal | 60 °C, 30 s | |
| 4Extend | 72 °C, 1 min | 72 °C, 1 min | |
| 5Final extension | 72 °C, 5 min | 72 °C, 5 min |
- v5Scientist9 Oct
Primer dimers at 58 °C.
| Change | Step | v3 18 Sep, Lab manager | v5 9 Oct, Scientist |
|---|---|---|---|
| 1Initial denaturation | 95 °C, 3 min | 95 °C, 3 min | |
| 2Denature | 95 °C, 30 s | 95 °C, 30 s | |
| ± | 3Anneal | 60 °C, 30 s | |
| ± | 4Extend | 72 °C, | 72 °C, 1 min |
| 5Final extension | 72 °C, 5 min | 72 °C, 5 min |
- v4Scientist2 Oct
Extension to 1 min.
- v5Scientist9 Oct
Primer dimers at 58 °C.
| Change | Step | v1 21 Aug, Lab manager | v5 9 Oct, Scientist |
|---|---|---|---|
| 1Initial denaturation | 95 °C, 3 min | 95 °C, 3 min | |
| ± | 2Denature | 95 °C, 30 s | |
| ± | 3Anneal | 60 °C, 30 s | |
| ± | 4Extend | 72 °C, | 72 °C, 1 min |
| 5Final extension | 72 °C, 5 min | 72 °C, 5 min |
- v2Scientist4 Sep
Weak bands at 55 °C.
- v3Lab manager18 Sep
Denature as the polymerase sheet says.
- v4Scientist2 Oct
Extension to 1 min.
- v5Scientist9 Oct
Primer dimers at 58 °C.
The lab’s week, newest first
A lab manager reads every change in one trail.
qPCR, standard curvev6
“Dilution series to 1:10, five points.”
Western blot transferv8
“Bands smeared at 100 V.”
Cell passagev12
“Confluent a day early, split at 80%.”
You write the why
Benchmark writes the rest. Nothing you type here leaves this page.
System What Benchmark writes
60 °C 62 °C
- Who
- You
- When
- to the second, on saving
- Version
- v5 to v6
- Why
- “Faint bands at 60 °C.”
What a lab asks first
- Who is Benchmark for?
- Research labs: the scientists who run protocols and the managers who keep them.
- What does a change record?
- Who made it, when, and why, on the version it made.
- Is an old version ever lost?
- No. Every version stays, to read beside any other.
- How does a lab start?
- Ask with a work email. Request access